Adult erectile functions and prepubertal role of vitamin C supplementation during crude oil-contaminated water ingestion
Shakiru Ademola Salami 1 * , Grace Temitope Omojuwa 1 , Michael Olabode Allen 1 , Hussein Mofomosara Salahdeen 1 , Babatunde Adekunle Murtala 1
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1 Lagos State University, College of Medicine, Department of Physiology, Ikeja, NIGERIA* Corresponding Author

Abstract

Background: The benefits of vitamin C treatment for erectile functions during prepubertal crude oil-contaminated water (CCW) ingestion are unknown. We currently considered the import of vitamin C supplement during prepubertal crude oil-contaminated water ingestion on adult erectile mechanisms in Wistar rats.
Materials and methods: 18 prepubertal male rats were divided into three groups and given saline, 2.5 ml CCW, and 2.5 ml CCW + vitamin C (10 mg/kg), separately, until adulthood. Cavernosa tissue responses to phenylephrine, acetylcholine, potassium chloride, and responses after incubation with glibenclamide, nifedipine, methyl blue, and indomethacin were investigated. Serum testosterone and the cavernosa oxidative biomarkers were determined.
Results: Testosterone and catalase activity were significantly reduced in CCW group, while malondialdehyde activity was significantly increased. Potassium significantly increased cavernosa contraction in CCW-treated group. Incubation of the cavernosa with nifedipine and indomethacin reduced the percentage of relaxation in CCW group. When compared to the vitamin C-supplemented groups, incubating the cavernosa with methyl blue and glibenclamide markedly reduced relaxation in CCW.
Conclusions: Prepubertal CCW ingestion impaired adult cavernosa ATP-sensitive K+ channels, receptor and voltage-operated calcium channels, guanylate cyclase, and prostaglandin activity. Vitamin C administration reduced contractile impairments by increasing antioxidant activity in the cavernosa.

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Research Article

J CLIN EXP INVEST, Volume 14, Issue 4, December 2023, Article No: em00829

https://doi.org/10.29333/jcei/13751

Publication date: 02 Oct 2023

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Article Downloads: 254

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