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Abstract
Background: Reference range of uric acid is narrow and at the cut-off of 7 mg/dl is defined as hyperuricemia, correct estimation of uric acid play an essential role in management of patient. The objective of this study was to find out the variation in serum uric acid values measured by two different analyzers, i.e., wet and dry chemistry.
Materials and method: Serum uric acid was measured in 227 blood samples received in clinical biochemistry laboratory for analysis of uric acid over a period of two months by two different instruments, i.e., wet chemistry instrument based on colorimetry method and dry chemistry instrument based on reflectance spectrophotometry.
Results: The mean difference of serum uric acid between two methods was 0.89 mg/dl which was statistically significant (t =12.92, p < 0.001) and Pearson correlation coefficient was 0.9688. The Bland-Altman (BA) plot analysis showed the maximum difference between wet and dry chemistry from -1.15 mg/dl to 2.93 mg/dl, with a mean difference of 0.89 mg/dl. Samples were further categorized on the basis of gender, out of 227 patients enrolled 142 (63%) were males and 85 (37%) females. The BA plot demonstrated limit of agreement ranging from -1.55 to 3.57 mg/dl in female patients and -0.83 to 2.48 mg/dl in male patients.
Conclusion: Good correlation exists between wet and dry chemistry, however the two methods are not similar. While interpreting the laboratory findings of uric acid, the method used must be checked carefully and during the follow up period switching of the method for uric acid estimation should be avoided.
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Article Type: Research Article
J CLIN EXP INVEST, Volume 16, Issue 1, March 2025, Article No: em00841
https://doi.org/10.29333/jcei/15685
Publication date: 01 Jan 2025
Online publication date: 05 Dec 2024
Article Views: 126
Article Downloads: 62
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