Can lactoferrin modulate the immunostimulant activity of levamisole in rats immunosuppressed by cyclophosphamide?
Wafaa Abdou Mohamed 1 *
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1 Dept. Clinical Pathology, Faculty of Veterinary, Zagazig University, 44511 Zagazig, Egypt* Corresponding Author

Abstract

Objective: The aim of this study was to study the immunomodulatory activity improvement of levamisole by using lactoferrin when applied to immunosuppressed rat model.
Methods: The study was designed as follows, 140 male albino rats (250-280 g) 14 weeks old were used in our work. Rats were randomly divided into seven groups, 20 in each. The group I was kept as a control, group II was given cyclophosphamide (CYP) at a single intraperitoneal dose of (250 mg/kg body weight), group III CYP and lactoferrin (Lac) treated group, group IV orally administrated Lac only (0.5%) in drinking water, group V treated with CYP and levamisole, group VI administrated levamisole orally at a dose of (2.5 mg/kg body weight) and group VII was given CYP, Lac and levamisole. Animals were sacrificed and two separate blood samples were collected after 21 days from the beginning of the experiment for measuring the total and differential leukocyte count, serum total proteins, albumin, alpha globulin, beta globulin and gamma globulin, Nitric oxide (NO) production and lysozyme activity.
Results: CYP group showed significant decrease in the above mentioned parameters, which were improved after administration of both lactoferrin and levamisole.
Conclusion: Our study concluded that lactoferrin improve the immunostimulant effect of levamisole in CYP- immunosuppressed rats.

License

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Article Type: Research Article

J Clin Exp Invest, 2014, Volume 5, Issue 1, 48-53

https://doi.org/10.5799/ahinjs.01.2014.01.0358

Publication date: 11 Mar 2014

Article Views: 2072

Article Downloads: 944

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